Figure 5

Effects of 17 β -oestradiol on LPCAT1 mRNA and protein expression and PAF release in prostate cancer cells. Cells were treated with 17β-oestradiol (10⁻⁷ M) in the presence or absence of RU486 (10⁻⁶ M), MPP (10⁻⁶ M) and cyclofenil (10⁻⁶ M) for 24 h. (A, C) LNCaP cells and (B, D) PC-3 cells. Values are presented as mean percent control±s.e.m. (A, B) or mean±s.e.m. (C, D) for five cultures performed in triplicate. *P<0.05 and **P<0.01 compared with control; ^#P<0.05 and ^##P<0.01 compared with 17β-oestradiol (10⁻⁷ mol/l⁻¹).