Figure 1

PCCL, together with TRIMEL and TNF-α, induces a fast differentiation of active APCs (TAPCells) from peripheral blood monocytes. (A) PBMCs from healthy donors (n=3) were incubated in supplemented AIM-V medium. Next, adherent cells were stimulated for 24 h with TNF-α (20 U ml^–1), TNF-α+TRIMEL (100 mg ml^–1) (Trimel), TNF-α+PCCL (100 mg ml^–1) (PCCL100), TNF-α+PCCL (90 mg ml^–1)+TRIMEL (10 mg ml^–1) (PCCL90+Trimel10) or TNF-α+PCCL (80 mg ml^–1)+TRIMEL (20 mg ml^–1) (PCCL80+Trimel20). AM, non-stimulated monocytes. Expression of MHC molecules and maturation markers were determined using flow cytometry over CD11c⁺ population. Graphics represent mean fold induction relative to AM (*P<0.05, t-test). (B) Stimulated (filled figures) and non-stimulated (clear figures) PBLs from healthy donors (n=3) were cultured with PCCL-loaded APCs (PCCL80+Trimel20) for 16 h. IFN-γ secretion was measured by ELISPOT assay. Experiments were performed in triplicate (*P<0.05, **P<0.01, ANOVA). Every symbol (circle, square and rhombus) represents a different donor.