Figure 1

Fluorescent immunohistochemical staining using the HistoRx AQUAnalysis platform. Representative fluorescence immunohistochemistry images for (A) sCAIX, (B) Ki67, and (C) BAX expression in OSCC samples. Upper and lower panels present examples of low and high biomarker expression, respectively. Merged images are pseudo-coloured blue for DAPI, green for pan-cytokeratin, red for the biomarker of interest, and greyscale for vimentin. AQUA scores for BAX and sCAIX and the percent positive tumour nuclear area for Ki67 are indicated in the merged images.