Figure 2

p53 dependency of apoptosis induction in ES cells after APR-246 treatments. (A) Three different cell lines (STA-ET-7: 1–3) generated from the same patient at different stages of the disease responded differently to APR-246 treatment. Results are representative of three independent experiments. Error bars denote s.e.m. P values represent statistical differences between the number of cells in treated vs untreated samples in each cell line. Middle panel shows an immunoblot depicting mutant p53 expression levels in each cell line while lower panel shows histograms of Licor-quantified bands relative to GAPDH. (B) siRNA-mediated knockdown of mutant p53 suppresses APR-246-induced apoptosis in the ES cell line STA-ET-7.2 as assessed by FACS analysis of Annexin-V+ (DAPI-negative) cells. n=3, error bars denote s.e.m. Lower panel: an immunoblot (middle) and histograms showing Licor quantification of immunoblot bands of total mutant p53 levels in STA-ET-7.2 cells transfected with control siRNA or p53 siRNA and treated with the stated concentration of APR-246.