Figure 4

Cell cycle perturbations induced by treatment with the IC50 doses of VX-680 and ZM447439 in drug-sensitive ( A ) and drug-resistant ( B ) human OS cell lines. Graphs were derived from cell cycle analyses performed after 48 h of drug treatment. The intensity of propidium iodide fluorescence (representative for the DNA content) is plotted on x axis. The intensity of the incorporated BrdU fluorescence (representative for the DNA synthesis) is plotted on y axis. The different cell populations were identified according to the scheme shown in the plot on the top left of panel A. CTR, control non-treated cells cultured in drug-free and DMSO-free medium; DMSO, control non-treated cells cultured in the presence of DMSO concentrations corresponding to those of drug-treated samples; VX-680 or ZM447439, cells treated with IC50 dosage of each Aurora kinase inhibitor drug.