Figure 2

Anchorage-independent growth and invasive potential of ccRCC cell line NRCC with altered expression of GSTM3 by stable transfection. (A) Knockdown of GSTM3 expression by shRNA. (B) Ectopic overexpression of GSTM3 by the transfection. (C) Knockdown of GSTM3 increased anchorage-independent growth. (D) Ectopic overexpression of GSTM3 decreased anchorage-independent growth. (E) Knockdown of GSTM3 increased invasive potential. (F) Ectopic overexpression of GSTM3 decreased invasive potential. (G) Cell cycle analysis by flow cytometry. *P<0.05.