Figure 3

Anchorage-independent growth and invasive potential of ccRCC cell lines 786-O and ACHN with altered expression GSTM3 by transient transfection. *P<0.05, **P<0.01. (A–D) Expression levels of GSTM3 in ACHN and 786-O cells after transient transfection with shRNA constructs and TOPO-GSTM3 construct, respectively, as detected by qRT-PCR and western blot. (E–H) Invasive potential ACHN and 786-O cells after the transient transfection, as detected by Transwell assay. (I–J) Anchorage-independent growth in ACHN cells after transient transfection with shRNA constructs and TOPO-GSTM3 construct, as detected by the double-layered soft agarose culture system. (K) Background level of GSTM3 expression in ACHN, 786-O and NRCC cells, as detected by western blot.