Figure 5 | British Journal of Cancer

Figure 5

From: An anti-leishmanial thiadiazine agent induces multiple myeloma cell apoptosis by suppressing the nuclear factor kappaB signalling pathway

Figure 5

DETT activates cell apoptotic pathway and suppresses expression of anti-apoptotic proteins. (A) Six MM cell lines were treated with 0 or 20 μ M of DETT for 24 h, followed by evaluation of PARP. (B) OCI-My5 and RPMI-8226 cells were treated with 0, 2.5, 5, 10, or 20 μ M of DETT for 24 h, and cell lysates were subject to caspase-3, caspase-8, PARP, and GAPDH analysis. (C) OCI-My5 and RPMI-8226 were treated with DETT for 24 h, followed by western blotting assay for pro-apoptotic (Bim) and anti-apoptotic (Bcl-2 and XIAP) proteins using specific antibodies. Abbreviations: Pro-casp3: pro-caspase 3; cle-casp8: cleaved caspase 8.

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