Figure 3

Validation of genes perturbed by CHKA silencing. RT-qPCR of selected up (A) and down (B) modulated genes in CHKA-silenced EOC cell lines. GAPDH was amplified as endogenous control, and I64-hTERT cells preparations were used as calibrator. The mean of five independent experiments is reported. (C) Evaluation of IL6 and IL8 release by bioplex assay on conditioned media from both EOC cell lines. Data are the mean±s.d. of three independent experiments.