Figure 5

CHKA silencing regulates cell migration and induces alteration in F-actin organisation. (A): Wound-healing assay: photographs of a representative experiment of three performed taken at the time of scratch (t=0) and at t=72 h. Magnification × 200: mean percentage±s.d. of migration at t=72 h is reported in the graph for each cell line. (B): Representative immunofluorescence staining of INTOV11 and SKOV3 CHKA-silenced and control cells. Cells were wounded 72 h after transfection and analysed 24 h later. F-actin filaments were visualised as green fluorescence through Alexa-488–phalloidin binding, and cell nuclei were stained by DAPI. Actin stress fibers and focal adhesion sites are indicated by red arrows (Magnification: × 300). In each panel, a picture of the wound border is reported in the inset (Magnification × 200).