Figure 5
Treatment with PL promotes autophagy induction. (A) Piperlongumine treatment results in LC3-II accumulation and decrease of ULK1 Ser757 phosphorylation. Piperlongumine induction of autophagy is ROS dependent, as NAC completely reverses effects of PL. Cells were treated with indicated concentrations of PL alone or in combination with 10 mM of NAC for 24 h. (B) Concomitant treatment of cells with PL (10 μ M) and Baf-A1 (10 μ M) results in higher accumulation of LC3-II then treatment with PL alone, which indicates PL acts as autophagy inducer rather than inhibitor. Total cellular lysates were subjected to western blotting with specific antibodies. Upper band (if visible) corresponds to LC3-I and lower band corresponds to LC3-II form of LC3 protein.
