Figure 2
Hypoxia induces Nur77 expression through the coordination between β -catenin and HIF-1 α. (A) HCT116 and SW480 cells were transfected with non-specific (NS) siRNA or HIF-1α siRNA. Cells were then incubated at 1% O2 or with 100 μ M DFX for 4 h and harvested for western blotting. (B) SW480 cells were transfected with expression vector encoding β-catenin and/or HIF-1α. Cells were then incubated at 1% O2 for 4 h and harvested for RT-PCR. (C) SW480 cells were transfected with NS siRNA or HIF-1α siRNA, empty vector or GFP-β-catenin as indicated for 24 h, followed by 4 h hypoxic incubation. 18s rRNA was used as an internal control for RT-PCR, whereas β-actin was used as a loading control for western blotting. The blots were quantified by densitometry and the values were normalised with 18r RNA or β-actin. All blots shown are representative of three independent experiments. Bar, ±s.d. *P<0.05.
