Figure 4
Nur77 enhances β -catenin stability independent of APC and p53. (A) SW480 cells transfected with empty vector or Myc-Nur77 for 24 h were treated with 100 μ M cycloheximide (CHX) under 1% O2 for the time indicated. Changes in β-catenin and Nur77 levels were detected by western blotting. β-Actin was included as a loading control. (B) Two paired cell lines were used: HCT116 and HCT116-p53−/− (both with mutant β-catenin), HT29-β-gal (with mutations in both p53 and APC) and H292-APC (with mutant p53 but expressing full-length APC). Cells were transfected with expression constructs as indicated for 24 h. Transfected cells were incubated with 100 μ M desferrioxamine (DFX) or 1% O2 for 4 h. For HT29 paired cells, 100 μ M zinc chloride was added to induce the expression of APC during starvation. β-Actin served as the loading control. Immunoblots were quantified by densitometry and the values were normalised with β-actin. All blots shown are representative of three independent experiments. Bar, ±s.d. *P<0.05.
