Table 1 Counting experiments of CellSearch CTC control samples on the DEPArray system

From: Semiautomated isolation and molecular characterisation of single or highly purified tumour cells from CellSearch enriched blood samples using dielectrophoretic cell sorting

  

FITC+ cells

APC+ cells

Experiment

Time between CS and DA experiment (days)

CS count

DA count

DA/CS%

CS count

DA count

DA/CS%

1

6

1026

766

75%

59

43

73%

2

1

1120

465

42%

70

26

37%

3

0

1142

567

50%

72

40

56%

4

0

1083

729

67%

81

47

58%

5

1

1032

454

44%

60

35

58%

6

5

1037

652

63%

41

26

63%

7

9

1070

823

77%

62

43

69%

8

15

1083

663

61%

51

33

65%

9

6

1197

898

75%

57

42

74%

10

5

973

656

67%

67

56

84%

Mean±s.d.

5±4

1076±61

667±137

62±12%

62±11

39±9

63±12%

CV

93%

6%

21%

20%

17%

23%

19%

Minimum

0

973

454

42%

41

26

37%

Maximum

15

1197

898

77%

81

56

84%

  1. Abbreviations: APC=allophycocyanin; CS=CellSearch CTC test; CTC=circulating tumour cell; CV=coefficient of variation; DA=DEPArray; FITC=fluorescein isothiocyanate.
  2. Results of 10 different experiments are summarised. The percentage of cells enumerated on the CellSearch system that could be re-identified on the DEPArray system was used as a measure to estimate overall cell loss during DEPArray cartridge loading.