Table 2 Multigene mutation analysis of single tumour cells and groups of tumour cells isolated with the DEPArray system from a CellSearch cartridge

Gene	Mutation	Control	Single cell 1	Single cell 2	Single cell 3	Pool of 10 cells
ARID1A	2830C>T	WT	WT	NC	NC	WT
BRAF	1391G>T	MUT	MUT	WT	MUT	MUT
KRAS	38G>A	MUT	MUT	MUT	MUT	MUT
NF2	691G>T	MUT	MUT	NC	MUT	MUT
PIK3CA	3140A>G	WT	WT	WT	WT	WT
PIK3CA	333G>C	WT	WT	WT	NC	WT
TP53	853G>A	WT	WT	WT	WT	WT
TP53	580C>T	WT	WT	NC	NC	WT
PIK3CA	1633G>A	WT	WT	WT	WT	WT
TP53	839G>A	MUT	MUT	MUT	MUT	MUT

Abbreviations: Control=unamplified DNA of 3–5 million MDA-MB-231 cells harvested from the same culture flask as the one used to perform the isolation of single cells and the group of tumour cells; MUT=mutant allele detected by the iPlex assay; NC=no call; WT=only wild-type allele detected by the iPlex assay.
The bold entries indicate that no reliable call could be obtained based on the iPlex analysis.

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