Figure 1

Effect of o-OPN on growth of 275-3-2 cells. OPN-expressing 275-3-2 cells were injected subcutaneously at 5 × 10⁴ cells per mouse. OPN was added to the drinking water at the indicated concentration starting 5 days later, and tumour size was measured every other day with calipers. (A) Tumour size in control and OPN-fed mice: results of a representative experiment. N=10 (water); 13 (0.3 mg ml⁻¹ OPN, days 9–17) or 8 (0.3 mg ml⁻¹ OPN days 19 and 21). (B) Mean tumour size of control and OPN-fed tumours on day 15; combined results from three independent experiments. N=30 (0 mg ml⁻¹ OPN) or 32 (0.3 mg ml⁻¹ OPN). (C) Tumour growth rate in mice fed different doses of OPN. (D) Mouse body weight during initiation of o-OPN feeding. Asterisk (*) indicates P<0.05 compared with control at the same time point.