Figure 3
OPN reactivity in plasma of mice fed OPN. bOPN, 50 mg per mouse, was dissolved in Yoo-Hoo chocolate milk (which contains bOPN) and administered orally in a bolus over a period of 15–20 min to OPN-deficient mice. Plasma was collected at different times after feeding and assayed for anti-OPN antibody reactivity using a competition ELISA. Data for both 3-week old (open bars) and 10-week old (filled bars) are shown. Inset: representative standard curve using Lacprodan-OPN10 as standard; N=3 per group YH=YooHoo. *P=0.05 as compared with time 0, determined by Mann–Whitney test.
