Figure 6
α9 Integrin is expressed in 275-3-2 tumours. (A) qPCR. RNA from 275-3-2 cells, several 275-3-2 tumours or from normal liver (positive control) was purified and cDNA prepared. qPCR was performed as described in Materials and Methods section and normalised to EF1a1. (B) Immunofluorescence was performed on cryosections from untreated 275-3-2 tumours for α9 integrin (blue) and CD-31 (red). Nuclei are stained green with Yo-Pro. Thin arrows indicate α9 expression closely associated with blood vessels; block arrow indicates α9 expression in non-vascular cells. Bar=50 μm.
