Figure 1
MiR-145 levels correlate inversely with metastatic capacity in CRC cell lines and CRC tissue. (A) RT–PCR data for miR-145 in seven human colorectal cell lines: U6 snRNA, loading control; NTC, no template control, n=3. (B) MiR-145 RT–PCR data in sublines with different metastatic capacity: U6 snRNA, loading control, n=3. (C) qPCR data for miR-145 levels in 43 pairs of primary CRC and matched normal colon epithelial tissues. (D) qPCR data of miR-145 levels in primary CRC (metastasis positive or metastasis free) lymph node and/or liver metastasis. The term; –ΔCt (–ΔCt=CtU6−CtmiR-145) is used to denote expression levels of miR-145. U6 snRNA was the internal normalised reference for miR-145. Error bars (s.d.) were calculated from triplicate samples.
