Figure 4
Detection of target genes regulated by miR-145 in CRC cell lines. (A) Luciferase activity after transfection with the four wild-type 3′-UTR constructs, miR-145, or miR-control. (B) miR-145-binding sites in the 3′-UTR region of Fascin-1;luciferase activity after transfection with mutant 3′-UTR constructs in Fascin-1. The no-insert control (NO), wild-type (WT), and mutated-type (MT) constructs are highlighted with the seed region underlined, and base substitutions are highlighted as bold text. (C) Luciferase assays using the mutated vectors in which the specific sites targeted by the miR-145 were deleted. MiR-145 expression levels affect Fascin-1 protein (D); and mRNA expression (E), in CRC cell lines. Error bars (s.d.) were calculated from triplicate samples. *P<0.05.
