Figure 2 | British Journal of Cancer

Figure 2

From: Restoration of PPP2CA expression reverses epithelial-to-mesenchymal transition and suppresses prostate tumour growth and metastasis in an orthotopic mouse model

Figure 2

PPP2CA overexpression causes reversal of EMT in PCa cells. (A) PPP2CA-overexpressing or PPP2CA-silenced PCa cells were grown on fluorodish, fixed and stained with Alexa Fluor 488-conjugated phalloidin. Images were taken under confocal microscope. Data show that low PPP2CA-expressing (C4-2-Neo and PC3-Neo and LNCaP-shPPP2CA) cells exhibited several filopodia- and lamellipodia-like projections (arrows) as compared with PPP2CA-overexpressing (PC3-PPP2CA and C4-2-PPP2CA and LNCaP-NT) cells. Expression of various epithelial (E-cadherin and cytokeratin-18) and mesenchymal (N-cadherin, vimentin, Slug and Twist) markers at (B) transcript and (C) protein levels was examined by qRT–PCR and immunoblot assays, respectively. Data are presented as fold change in expression upon PPP2CA overexpression and silencing. Bars represent mean ±s.d., n=3.

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