Figure 1 | British Journal of Cancer

Figure 1

From: Small molecule MIRA-1 induces in vitro and in vivo anti-myeloma activity and synergizes with current anti-myeloma agents

Figure 1

MIRA-1 demonstrated potent anti-myeloma activity in vitro. (A) Chemical structures of MIRA-1; Chemical name: 1-[(1-Oxopropoxy)methyl]-1H-pyrrole-2,5-dione. Molecular formula: C8H9NO4 (B–E). (B) MM cell lines expressing wild-type p53 (MM.1S, H929) or mutant p53 (LP1, U266, and 8226) were incubated with MIRA-1 (0–20 μmol l−1), and viability was determined at 48 h using MTT assay. (C) MIRA-1-induced cytotoxicity in primary MM samples from newly diagnosed MM patients. Myeloma cells isolated from the bone marrow via CD138+ selection were cultured with MIRA-1 (0–20 μmol l−1), and viability was assessed by MTT at 48 h. (D) PBMCs and (E) BMMNCs were treated similarly with MIRA-1 and cyototoxicity was assessed by MTT assay. Viability of the cells was expressed as percentage of the DMSO-treated control. Data represents means (±s.d.) of triplicate cultures.

Back to article page