Figure 2

MIRA-1 induced apoptosis of MM cells irrespective of p53 status. (A) MM.1S, H929, 8266, and LP1 cells were treated with MIRA-1 (0–20 μmol l⁻¹) or DMSO for 48 h. Apoptosis was measured by Annexin V and PI binding assay by flow cytometry. Apoptotic cells were quantified after normalising with DMSO treated cells. (B) MIRA-1 inhibits colony formation in MM cells. MM.1S or LP1 cells (5 × 10⁴ cells ml⁻¹) treated with MIRA-1 or DMSO were used in forming cell layer in methyl cellulose-based medium. Colonies were counter 14 days later. (C) MIRA-1 inhibits the migration of MM cells. MM.1S or LP1 cells treated with DMSO or MIRA-1 were analysed for their migration ability. The migration assay was performed using a 24-well transwell plate and the migration cells were quantified as described in Materials and Methods. Data are expressed as mean ± S.D. of triplicate cultures. *P<0.05, **P<0.01.