Figure 2

Rac1 expression in HNSCC cells. Western blotting analysis was done using protein extracts from parental FaDu, SCC25, CAL27 and treatment-resistant FaDu-IRR, SCC25-IRR and CAL27-IRR cells, as described in Materials and methods section. α-Tubulin was used as a loading control. IDV was calculated for each protein band and normalised to the α-tubulin band density after background correction. IDV ratio means fold increase of Rac1 band density in IRR cells to those in parental HNSCC cells. Western blots shown are representative of three independent experiments.