Figure 4
BI-69A11 suppresses the migration and invasion of colon cancer cells. (A) Microscopic observation of the growth of HCT116 and HT29 cells after sub-lethal dose of BI-69A11 exposure for the indicated times (h). A ‘wound’ was created with a pipette tip before treatment with 0.5 μ M BI-69A11 containing culture medium. The data are representative of three independent experiments. Photographs were taken at 4 × magnification. (B) Quantification of wound-healing results. Data are means±s.e. of three random widths along the wound with time. *P<0.05, **P<0.01, ***P<0.001 represent level of significance with respect to control. (C) Data represent the average percentage of cells (±s.d.) invading the Boyden chamber inserts coated with Matrigel from three independent experiments, each performed in triplicate; *** represents P<0.0001. For this experiment HT29 cells were treated with 0.5 μ M of BI-69A11.
