Figure 5
From: Prevention of multidrug resistance (MDR) in osteosarcoma by NSC23925
Pgp activity in different cell sublines. (A) Drug intracellular accumulation images of U-2OS cell sublines incubated with Calcein AM and R123. The U-2OS/paclitaxel0.006–NSC23925 cells demonstrated a significantly higher accumulation of Calcein AM and R123 as compared with U-2OS/paclitaxel0.2 cells. (B) Calcein AM accumulation and R123 accumulation and efflux level in U-2OS cell sublines analysed by Microplate Spectrofluorometer. The U-2OS/paclitaxel0.006–NSC23925 cells showed a statistically higher accumulation of Calcein AM, R123 and lower relative efflux level of R123 than U-2OS/paclitaxel0.2 cells. Cells cultured with NSC23925 alone had no obvious effect on Pgp activity. Data are presented as mean±s.d. and analysed using Student’s t-test (two tailed, unequal variance). Statistical comparisons between selected and parental cells are presented: *P<0.05, **P<0.005, ***P<0.001.
