Figure 3
From: Connexins and cyclooxygenase-2 crosstalk in the expression of radiation-induced bystander effects
The effect of COX-2 on radiation-induced bystander effects mediated by connexin channels. Micronucleus formation in bystander normal human AG1522 fibroblasts cocultured with contiguous HeLa cells with normal (control) or knockdown levels of COX-2. HeLa parental cells (A and B) and HeLa cells expressing Cx26 (C and D) or Cx32 (E and F) with normal or knockdown levels of COX-2 were exposed to a mean absorbed dose of 50 cGy from 3.7 MeV α particles or were sham-treated. Within 5 min after exposure, they were intimately cocultured with AG1522 fibroblasts for 6 h (left panels) or 24 h (right panels). After coculture, the bystander cells were harvested and subjected to the micronucleus formation assay.
