Figure 4
From: Connexins and cyclooxygenase-2 crosstalk in the expression of radiation-induced bystander effects
The effect of COX-2 knockdown on junctional communication between HeLa Cx26 cells and AG1522 fibroblasts. HeLa Cx26 cells loaded with Calcein AM (green fluorescence) and CellTracker Orange (red fluorescence) were mixed with non-loaded AG1522 cells at a respective ratio of 1 : 99. Cells were microscopically examined with Texas Red (A) or FITC filters (B). (A) Representative field showing the loaded cells present in the coculture. (B) Same field in (A) showing Calcein AM dye transfer that occurs between HeLa Cx26 cells with normal or knockdown level of COX-2 and AG1522 fibroblasts at 2 h and 3.5 h after coculture.
