Figure 2
The effects of overexpression of HOXD10 in low-HOXD10-expressing OPL and HNSCC cells. (A). Expression of HOXD10 assessed using qPCR and WB, confirming raised expression in the transfected cells, compared with empty vector-transfected controls. β-actin is used as a loading control in WB. (B–D). Increasing HOXD10 expression in D19 and B22 increases migration in the Transwell assay (B), increases adhesion to fibronectin (C) and increases proliferation as assessed by MTS assay over 96 h (D). (E) Increasing HOXD10 expression in D19 and B22 reduces invasion into matrigel. *P<0.05, ***P<0.001. Each assay was conducted in triplicate and repeated three times. The data show the mean of triplicate repeats±s.e.m.
