Figure 6
AMOT-p80 exerts similar phenotypic effects to that seen on manipulation of HOXD10. (A) Expression of AMOT-p80 assessed using qPCR and WB, confirming raised expression of AMOT-p80 in transfected cells, compared with empty vector-transfected controls. β-actin is used as a loading control. (B) Cell growth was assessed using the MTS assay over 96 h. Introducing AMOT into low-AMOT cells (D19, B22) has resulted in an increase in their proliferation. (C) D19 and B22 cells transfected with AMOT show an increase in migration in the Transwell assay compared with cells transfected with a control plasmid. (D) Assessing cell adhesion to a fibronectin-coated surface shows that introducing high levels of AMOT by transfection had no significant effect. *P<0.05, ***P<0.001, each assay was conducted in triplicate and repeated three times. Data show the mean of triplicate repeats±s.e.m.
