Figure 1
From: COUP-TFII regulates metastasis of colorectal adenocarcinoma cells by modulating Snail1
Chicken ovalbumin upstream promoter-transcription factor II and Snail1 are overexpressed in colon adenocarcinoma tissue. (A) Expression of COUP-TFII and Snail-1 in human colorectal adenocarcinoma tissue. Western blots were used to determine expression of COUP-TFII and Snail1 in human colorectal adenocarcinoma tissue. Lanes contain equal amounts of nuclear protein from colorectal adenocarcinoma tissue (T) and paired normal tissue (N). (B) Quantification of image density from A. *P<0.01 compared to normal. (C) Representive images were shown for the expression of COUP-TFII and Snail1 in 326 human colorectal adenocarcinoma tissue samples, determined by western blot. (D) Survival curves for colorectal adenocarcinoma patients with either high or low COUP-TFII (P<0.01). (E) Human colon adenocarcinoma LOVO or HT29 cells stably expressing control vector (CON), shRNA against COUP-TFII (COUP-TFII KD), shRNA against Snail1 (Snail-1 KD), COUP-TFII KD + Snail-1 overexpressing (Snail-1 OV), COUP-TFII overexpressing (COUP-TFII OV), or COUP-TFII OV + Snail-1 KD were cultured. Expression of COUP-TFII and Snail1 by western blot. Tubulin was the loading control. Density of bands were quantified.
