Table 1 Conditioned medium from BRAFV600E tumour cells exhibits increased cytokine and MMP-1 levels

From: BRAFV600E melanoma cells secrete factors that activate stromal fibroblasts and enhance tumourigenicity

Human multiplex cytokine and MMP-1 levels in Bowes cells

 

IL-1 β (pg ml−1)

IL-6 (pg ml−1)

IL-8 (pg ml−1)

MMP-1 (ng ml−1)

Empty vector clone 8

CM

0.12

239.42

0.51

ND

CM & PLX

0.08

171.12

0.08

ND

mBRAF clone 7

CM

5.31

329.83

9.76

ND

CM & PLX

3.28

98.4

2.83

ND

mBRAF clone 21

CM

1.98

400.72

51.73

19.09

CM & PLX

1.01

194.22

21.83

10.32

  1. Abbreviations: CM=conditioned medium; IL=interleukin; MMP-1=matrix metalloproteinase-1. Conditioned medium from Bowes clones 7, 8, and 21 was collected and subjected to a human multiplex cytokine assay. Matrix metalloproteinase-1 levels were not detected (ND) for clones 7 and 8.
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