Table 2 Tumour explant medium collected from BRAFV600E tumours shows heightened cytokine and MMP-1 levels

From: BRAFV600E melanoma cells secrete factors that activate stromal fibroblasts and enhance tumourigenicity

Human multiplex cytokine and MMP-1 levels in tumour explant media

 

IL-1 β (pg ml−1)

IL-6 (pg ml−1)

IL-8 (pg ml−1)

MMP-1 (ng ml −1 of tumour)

Empty vector clone 8

0.42±0.05

225.01±50.06

9.52±0.49

ND

mBRAF Clone 7

6.77±0.07

137.58±56.09

8.12±0.73

26.5±26.5

mBRAF Clone 21

1.02±0.41

478.67±116.09

14.79±3.73

538.27±108.88

  1. Abbreviations: IL=interleukin; MMP-1=matrix metalloproteinase-1; ND=not detected. Tumours were isolated from mice and a portion of each tumour was weighed and placed in serum-free culture medium for 48 h. Data are an average of three tumours for empty vector clone 8, three tumours for mBRAF clone 7, and seven tumours for mBRAF clone 21.
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