Figure 2
Loss of fascin expression sensitised breast cancer cells to chemotherapy in xenograft tumour model. (A–D) Fascin-positive (ShCon) and negative (ShFascin) MDA-MB-231 breast cancer cells were injected into nude mice as described in the Materials and Methods. The tumour volumes were measured following treatment with or without three cycles of doxorubicin. (A) Representative images showing tumour formed in nude mice after injection with ShCon or ShFascin cells. (B) Show mean±s.d. Tumour volumes in nude mice injected with ShCon cells and (C) show mean±s.d. tumour volumes in nude mice injected with ShFascin cells. The arrows indicate the time of doxorubicin injection. (D) ShCon (top) or ShFascin (bottom) xenograft tumours were collected from mice that received doxorubicin treatment at the end of the experiment and single-cell suspensions were prepared. Apoptotic cell death was detected by Annexin V and DAPI staining as described in the Materials and Methods. * Numbers on the right of the dot plot represent the total percentage of apoptosis (Q2+Q4).
