Figure 3
Fascin-positive breast cancer cells are more resistant to chemotherapy-mediated apoptosis. (A) Show representative dot plot of Annexin V and DAPI staining following treatment of ShCon (top) or ShFascin (bottom) breast cancer cells without (left) or with 200 ng ml−1 of doxorubicin (middle) or taxotere (bottom) for 24 h. *Numbers on the right of the dot plot represent the total percentage of apoptosis (Q2+Q4). Bar graphs showing mean±s.d. viable cells following treatment with increased doses of either doxorubicin (B) or taxotere (C) and data is representative of triplicate experiments. (D) Bar graphs showing mean±s.d. viable cells of triplicate experiments following treatment of T47-D cells with doxorubicin. T47-D is the parental T47-D cells, which are fascin negative. Fascin expression are T47-D cells that expressed wild-type fascin.
