Figure 5
Resistance of fascin-positive cells to apoptosis is primarily mediated via the Akt pathway. (A and B) ShCon or ShFascin breast cancer cells were pre-incubated in the presence or absence of AKT IV inhibitor (2 μ M) for 24 h followed by treatment with 200 ng ml−1 of doxorubicin for 48 h. (A) Western blot showing the expression levels of Akt, p-Akt and GAPDH in ShCon and ShFascin cells following treatment with AKT IV inhibitor. (B) Cells were harvested and apoptotic cell death was determined as above. Results showing mean±s.d. viable cells following treatment with doxorubicin and data is representative of triplicate experiments. *P<0.05.
