Figure 1
Analysis of miR-183 cluster levels in specimens. (A) qRT–PCR data showing miR-183 levels in oesophageal squamous cell carcinoma (ESCC) tissues and adjacent normal oesophageal tissues: T, ESCC tissues; N, normal oesophageal tissues; n=32, **P<0.005. (B) miR-183 expression in oesophageal intraepithelial neoplasia tissues and adjacent normal oesophageal tissues; LG-IEN, low-grade intraepithelial neoplasia tissues; HG-IEN, high-grade intraepithelial neoplasia tissues; n1=9, P>0.05; n2=21, **P<0.005. (C) miR-182 expression in ESCC tissues and adjacent normal oesophageal tissues; n=32, ***P<0.001. (D) miR-182 expression in oesophageal intraepithelial neoplasia tissues and adjacent normal oesophageal tissues; n1=9, P>0.05; n2=21, P>0.05. (E) miR-96 expression in ESCC tissues and adjacent normal oesophageal tissues; n=32, P>0.05. (F) miR-96 expression in esophageal intraepithelial neoplasia tissues and adjacent normal oesophageal tissues; n1=9, P>0.05; n2=21, P>0.05. The terms ΔCt(T/IEN/N)=CtmiRNA–CtU6, ΔΔCt=ΔCt(T/IEN)–ΔCt(N), and 2−ΔCt(T/IEN/N) are used to denote the level of miRNA in T/IEN/N tissues, 2−ΔΔCt is used to denote the relative level of miRNA in T/IEN tissues compared with N tissues. U6 snRNA was used as an internal control.
