Figure 3

MiR-183 targets PDCD4 in ESCC cells. (A) Bioinformatics tools were used to predict the binding region of miR-183 in the PDCD4-3′-UTR. (B) Sequence analyses indicated the miR-183 target sequences for different species. (C) miR-183 and si-PDCD4 expression affects PDCD4 protein expression in Eca109 and TE13 cells (D) but does not affect PDCD4 mRNA expression (E) in ESCC cells. (F) Luciferase activity after transfection with wild-type (WT)/mutant-type (MUT) 3′-UTR constructs in PDCD4. Error bars (s.e.m.) were calculated based on triplicate samples (**P<0.005, *P<0.05).