Figure 2
Cell proliferation induced by SOX2 overexpression via upregulation of cyclin B in vitro. (A) Expression of SOX2 in control cells and in SOX2-overexpressing SNU1041 and FaDu cells analysed using western blotting. (B) The in vitro growth rate of control and SOX2-overexpressing SNU1041 and FaDu cells (n=3). **P<0.01. After cells (2 × 104) were plated and grown in media, the total number of cells was counted every 2 days. (C) Expression of cyclin A, cyclin B1, cyclin D1, and cyclin E mRNAs in control and in SOX2-overexpressing cells was determined by means of quantitative RT-PCR. **P<0.01. (D) Induced protein levels of Cyclin B1 in SOX2-overexpressing SNU1041 and FaDu cells according to western blot analysis. (E) The suppressed expression of Cyclin B1 in SOX2-overexpressing SNU1041 cells that were transiently transfected with siCyclin B1, confirmed by western blot analysis. (F) The in vitro growth rate of SOX2-overexpressing SNU1041 cells that were transiently transfected with either scrambled siRNA or siCyclin B1 (n=3). **P<0.01.
