Figure 4
Gossypol in combination with TMZ inhibits GBM cell invasion in vitro. U87MG-luc2 spheroids implanted in collagen gel were exposed to gossypol±TMZ treatment over 15 days. Representative images of (A) control, (B) TMZ (100 μ M)-, (C) gossypol (3 μ M)-, (D) gossypol (3 μ M)/TMZ (100 μ M)-, (E) gossypol (100 μ M)-, (F) gossypol (6 μ M)/TMZ (100 μ M)-, (G) gossypol (12 μ M)- and (H) gossypol (12 μ M)/TMZ (100 μ M)-treated spheroids were taken at day 15. Scale bar: 1000 μm ( × 40 magnification). Invasion activity of U87MG-luc2 cells exposed to the absence or presence of (I) gossypol (3 μ M)/TMZ (100 μ M), (J) gossypol (6 μ M)/TMZ (100 μ M) and (K) gossypol (12 μ M)/TMZ (100 μ M) over 15 days of treatment, and (L) average invasion distance per day quantified as a percentage of control were quantified. Data presented are mean invasion distance±s.e.m. of three independent experiments, *P<0.05 compared with the control, #P<0.05 compared with TMZ treatment, ##P<0.05 compared with gossypol treatment, linear mixed model and ANOVA, post-hoc Tukey.
