Figure 2 | British Journal of Cancer

Figure 2

From: B-RAF mutation and accumulated gene methylation in aberrant crypt foci (ACF), sessile serrated adenoma/polyp (SSA/P) and cancer in SSA/P

Figure 2

Analysis for B-RAF codon 600 and K-RAS codon 12 mutations in ACF, SSA/P, and cancer in SSA/P. Point mutations of B-RAF codon 600 and K-RAS codon 12 were examined using the 2-step PCR–RFLP method. (A) B-RAF mutations in ACF, SSA/P, and cancer in SSA/P. The HT-29 colon cancer cell line, which is known to have a B-RAF mutation, was used as a positive control. Normal colonic mucosa was used as a negative control. (B) K-RAS mutations in ACF, SSA/P, and cancer in SSA/P. The LS174T colon cancer cell line, which is known to have a K-RAS mutation, was used as a positive control. A1 represents ACF from case 1. S1 and C18 represent SSA/P and cancer in SSA/P from case 1 and case 18, respectively.

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