Figure 1
From: New small molecules, ISA27 and SM13, inhibit tumour growth inducing mitochondrial effects of p53
Effects of ISA27 on tumour cell growth in vitro. (A) The effect of ISA 27 on p53 and cleaved caspase 3 levels were evaluated in whole lysates from different tumour cell lines by western blot. In all MCF-7, BHT-101 and KAT-4 cells, the treatment with ISA 27 increases p53 levels and consequently leads to an increase of apoptotic signalling. Actin was used as loading control. Images are representative of three independent experiments. (B, C) KAT-4 cells were treated with ISA27 for 24 and 48 h and cell proliferation was analyzed (B). ISA27 reduces cell proliferation in a time-dependent manner. Results are the mean of five independent experiments and are presented as mean±s.e.m. This effect was confirmed by analysis of DNA synthesis by means of tritiated thymidine incorporation (C). DNA synthesis was reduced by ISA27 treatment (*P<0.05 vs control 24H; **P<0.05 vs control 48H). Results are the mean of five independent experiments and are presented as mean±s.e.m.
