Figure 1
From: It is not all about BRCA: Cullin-Ring ubiquitin Ligases in ovarian cancer
Cullin-Ring ubiquitin Ligases (CRLs) are prototypical multi-subunit ubiquitin ligases. This figure presents the assembly of a prototypical multi-subunit E3 through adaptors. Substrates contain a degron that serves as a signalling platform for the recruitment by the E3. Rbx1/2 are recruited by cullins to bridge the substrate selected by the substrate recognition protein to the E2. E2 and E1 provide activated ubiquitin molecules that are coupled with target lysine residues within the substrates. Poly-ubiquitinated substrates are degraded by the proteasome. NAE (Nedd8-activating enzyme) catalyses the transfer of Nedd8 to cullins. Chemical approaches to target the CRLs are outlined.
