Figure 1

EGR3 expression in human prostate cell lines. (A) The EGR3 gene expression values were retrieved from GEO data set GDS3155 that contains expression data for 15 human prostate epithelial cell lines (note that the stroma cell line WPMY present in the data set was excluded from this figure). (B) Box plot of the mRNA expression of EGR family members in this data set, reflecting high variability (EGR1) and low variability of expression (EGR2–4). (C) EGR3 protein expression in a few prostate cell lines. Untreated cells were lysed and protein expression was analysed by western blot using anti-EGR3 antibodies. The EGR3 protein migrates at ∼50 kDa. Membranes were reprobed with anti-actin antibodies as loading control. (D) Comparison of EGR3 mRNA expression in M12 and P69 cells. Total RNA was extracted from untreated cells and EGR3 expression was measured by quantitative RT–PCR. Results are expressed relative to expression in P69 cells. (E) Subcellular localisation of EGR3 in M12 prostate cancer cells. Untreated prostate cancer cells M12 were lysed and fractionated using a commercial kit, followed by western blot analysis of protein levels using anti-EGR3 antibodies.