Figure 2 | British Journal of Cancer

Figure 2

From: MRI reveals the in vivo cellular and vascular response to BEZ235 in ovarian cancer xenografts with different PI3-kinase pathway activity

Figure 2

Levels of activated Akt are elevated in TOV-21G cells and decrease in xenografts after BEZ235 treatment, whereas the levels are low in TOV-112D cells and unchanged in xenografts by treatment. (A) Immunoblots show that levels of pAkt (Ser473) are higher in cell extracts from untreated TOV-21G compared with TOV-112D. The level of β-actin served as a control for protein loading. (B) Levels of p-S6 (Ser 235/236) and total S6 in cell extracts from untreated cells are higher in TOV-21G cells compared with TOV-112D. Numbers indicate relative protein levels adjusted for loading. (C) Immunostaining of tissue sections shows that pAkt levels are lower in BEZ235-treated TOV-21G xenografts compared with controls. The TOV-112D cells have low levels in pAkt that are unchanged by treatment. Adjacent tumour sections for each tumour served as negative (neg.) controls and displayed a very low signal intensity. The scale bars represent 1 mm.

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