Figure 5

Live cell imaging of pHGG cell lines reveals differences in migration velocity alongside changes in polarity and morphological appearance following treatment with LiCl and BIO. Paediatric glioma cell lines SF188 and KNS42 were incubated with 20 mM LiCl, 5 μ M BIO or growth medium and time lapse imaging was performed for 24 h using the Nikon Biostation IM time lapse imaging system. Stills taken at time 0 h and 24 h from movies of SF188 (A) and KNS42 (B) treated with either 20 mM LiCl, 5 μ M BIO or growth medium demonstrate changes in the morphological appearance of individual cells following treatment. Quantification analysis of distance (C) displacement (D) and velocity (E) from live cell imaging of both cell lines following treatment with 20 mM LiCl or 5 μ M BIO. Error bars are expressed as ±s.e.m. *P<0.05 by ANOVA for each cell type and treatment. Tracking analysis of SF188 (F) and KNS42 (G) from live cell imaging following treatment with 20 mM LiCl or 5 μ M BIO demonstrates differences in motility between cell lines and treatments.