Figure 2

The P2M3C cells exhibit increased expression and secretion of CXCL10 as compared with P2M5B cells. (A) Heat map of 30 genes differentially expressed between P2M5B and P2M3C cells grown in tissue culture (in vitro: grey line) and as lung metastases (in vivo: green line). Yellow indicates high expression, whereas red denotes low expression. The CXCL10 is indicated by a black arrow. (B) The mRNA expression of CXCL10 as determined by Illumina Mouse WG6v2 gene expression signal intensity values. (C) Densitometry map of secreted murine cytokines produced by pooled samples of P2M5B (blue bar) and P2M3C (red bar) cells in vitro and in vivo as measured using Mouse Cytokine Antibody Array Panel A. The size and density of each dot correlates with the amount of secreted cytokine. The CXCL10 is indicated by a yellow arrow. (D) Quantification of serum CXCL10 protein production relative to in assay controls (standardised density) for P2M5B (blue bar) and P2M3C (red bar) cells grown in vitro (grey line) and in vivo (green line). Data represent mean±s.e.m. (n=3). *P⩽0.05. **P⩽0.001.