Figure 3
From: Oncogenic CXCL10 signalling drives metastasis development and poor clinical outcome
Suppression of the CXCL10/CXCR3 axis reduces lung metastatic colonisation. (A) Stable shRNA-mediated suppression of CXCL10 and CXCR3 as confirmed by western blot analysis. Two shRNA constructs (KD #1 and KD #2) were tested for each gene of interest. Nontargeting shRNAs served as controls. β-Actin served as a protein control. (B) Reduction in serum CXCL10 concentration following shRNA-mediated suppression of CXCL10 in P2M3C and P2M5B cells as measured by ELISA. Quantification of surface lung colonies 3 weeks after tail vein injection in C57BL/6 mice of P2M3C and P2M5B cells transfected with shRNA targeting CXCL10 (C) or CXCR3 (D). Insets display representative images of lung colonisation. *P⩽0.05. **P⩽0.01.
