Figure 3

Downregulation of Ecm29 expression by miR-221/222 in PC3 and DU145 cells. (A) Ecm29 mRNA expression 72 h after transfection with miR-221/222. GUSB was used as an internal control. *P<0.0001. (B) Ecm29 protein expression 48 h after transfection with miR-221/222. GAPDH was used as a loading control. (C) miR-221/222-binding sites in Ecm29 mRNA. Luciferase reporter assays were carried out using a vector encoding the putative miR-221/222 target site in the Ecm29 3’-UTR (position 97–103) for wild-type and deletion constructs. *P<0.0001. Experiments were performed triplicate. The bars mean s.d.