Figure 4

Effects of silencing Ecm29 mRNA and protein expression by si-Ecm29 transfection in PCa cells on cell proliferation, migration, and invasion in PCa cell lines. (A) Ecm29 mRNA expression was determined at 72 h after transfection with si-Ecm29. GUSB was used as an internal control. (B) Ecm29 protein expression was evaluated by western blotting at 48 h after transfection with si-Ecm29. GAPDH was used as a loading control. *P<0.0001. (C) Cell proliferation was determined by XTT assays. (D) Cell migration activity was determined by wound healing assays. (E) Cell invasion activity was determined by Matrigel invasion assays. *P<0.0001. mock: untransfected cells, control: control siRNA-transfected cells. Experiments were performed triplicate. The bars mean s.d.